تاثیر عصاره اتانولی گیاه توکریوم پولیوم بر کلنیزاسیون کاندیدا گلابراتا در کبد و طحال و کلیه ها

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشگاه آزاد اسلامی واحد فلاورجان

2 استادیار،گروه میکروبیولوژی، دانشگاه آزاد اسلامی، واحد فلاورجان، اصفهان، ایران

چکیده

توکریوم پولیوم یک گیاه دارویی است که گونه های آن بیش از دو هزار سال است که در طب سنتی استفاده می شود. در این تحقیق تاثیر عصاره اتانولی گیاه توکریوم پولیوم بر کلنیزاسیون کاندیدا گلابراتا در کبد و طحال وکلیه ها بررسی گردید.
مواد و روش ها: در این مطالعه تجربی 90 سر موش ماده از نژاد NMRI در 9 گروه 10 تایی تقسیم شدند. گروه شاهد، نرمال سالین، گروه کاندیدایی با دریافت سوسپانسیون مخمر و گروه های تیمار آلوده به کاندیدا، عصاره اتانولی توکریوم پولیوم را به طور جداگانه با دوزهای 50، 100 و 200 میلی گرم بر کیلوگرم به صورت یک روز در میان و به مدت 20 روز، دریافت کردند. در پایان آزمایش، مایع هموژنیزه اندام های کبد، طحال و کلیه بر روی محیط کشت سابورو دکستروز آگار کشت و شمارش کلنی انجام شد.
یافته ها: بر اساس نتایج این تحقیق نمونه کبد تحت تیمار با کاندیدا- عصاره با دوز 100 میلی گرم بر کیلوگرم و نمونه های طحال و کلیه در دوز 200 میلی گرم بر کیلوگرم هیچ کلنی مشاهده نشد.
بحث و نتیجه گیری: بر اساس نتایج تحقیق، گیاه توکریوم پولیوم، تاثیر قابل توجهی در ایمنی بر علیه کاندیدا گلابراتا دارد.
کلمات کلیدی: توکریوم پولیوم، ، کاندیدا گلابراتا، کبد، طحال، کلیه ها

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The effect of ethanolic extract of Teucrium polium on Candida glabrata colonization in the liver, spleen and kidneys

نویسندگان [English]

  • maryam alsadat zarei 1
  • Mahboubeh Madani 2
چکیده [English]

Background: Teucrium polium is a medicinal plant which species have been used for over 2000 years in traditional medicine. The aim of this study was evaluation of the effect of ethanolic extract of Teucrium polium on Candida glabrata colonization in mice tissue.
Material and Method: The study was performed on 90 mature male mices (9 per group), that were devided into 10 groups: normal, placebo, 3 treatment group (candida- extract), control positive (Candida glabrata) and control negative (extract). Treatment groups were received 50,100 and 200 mg/kg dosages of extract for 20 days (every other day) by intraperitoneal injection. At the end of injections, homogenized liver, spleen and kidney were cultured on SDA medium and then colony count were performed.
Results: The results showed, that liver in 100 mg/kg, spleen and kidney in 200mg/kg haven’t any yeast.
Discussion and Conclusion: According to the results, Teucrium polium extract, perhaps can stimulat the immunity against Candida glabrata.

Keywords: Teucrium polium, Candida glabrata, liver, spleen, kidney

کلیدواژه‌ها [English]

  • Teucrium polium
  • Candida glabrata
  • Liver
  • spleen
  • kidney
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دوره 25، شماره 1
فروردین و اردیبهشت 1397
صفحه 151-160
  • تاریخ دریافت: 06 اردیبهشت 1396
  • تاریخ بازنگری: 19 مرداد 1396
  • تاریخ پذیرش: 09 خرداد 1397
  • تاریخ اولین انتشار: 09 خرداد 1397