تاثیر عصاره اتانولی گیاه توکریوم پولیوم بر کلنیزاسیون کاندیدا گلابراتا در کبد و طحال و کلیه ها

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشگاه آزاد اسلامی واحد فلاورجان

2 استادیار،گروه میکروبیولوژی، دانشگاه آزاد اسلامی، واحد فلاورجان، اصفهان، ایران

چکیده

توکریوم پولیوم یک گیاه دارویی است که گونه های آن بیش از دو هزار سال است که در طب سنتی استفاده می شود. در این تحقیق تاثیر عصاره اتانولی گیاه توکریوم پولیوم بر کلنیزاسیون کاندیدا گلابراتا در کبد و طحال وکلیه ها بررسی گردید.
مواد و روش ها: در این مطالعه تجربی 90 سر موش ماده از نژاد NMRI در 9 گروه 10 تایی تقسیم شدند. گروه شاهد، نرمال سالین، گروه کاندیدایی با دریافت سوسپانسیون مخمر و گروه های تیمار آلوده به کاندیدا، عصاره اتانولی توکریوم پولیوم را به طور جداگانه با دوزهای 50، 100 و 200 میلی گرم بر کیلوگرم به صورت یک روز در میان و به مدت 20 روز، دریافت کردند. در پایان آزمایش، مایع هموژنیزه اندام های کبد، طحال و کلیه بر روی محیط کشت سابورو دکستروز آگار کشت و شمارش کلنی انجام شد.
یافته ها: بر اساس نتایج این تحقیق نمونه کبد تحت تیمار با کاندیدا- عصاره با دوز 100 میلی گرم بر کیلوگرم و نمونه های طحال و کلیه در دوز 200 میلی گرم بر کیلوگرم هیچ کلنی مشاهده نشد.
بحث و نتیجه گیری: بر اساس نتایج تحقیق، گیاه توکریوم پولیوم، تاثیر قابل توجهی در ایمنی بر علیه کاندیدا گلابراتا دارد.
کلمات کلیدی: توکریوم پولیوم، ، کاندیدا گلابراتا، کبد، طحال، کلیه ها

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of ethanolic extract of Teucrium polium on Candida glabrata colonization in the liver, spleen and kidneys

نویسندگان [English]

  • maryam alsadat zarei 1
  • Mahboubeh Madani 2
چکیده [English]

Background: Teucrium polium is a medicinal plant which species have been used for over 2000 years in traditional medicine. The aim of this study was evaluation of the effect of ethanolic extract of Teucrium polium on Candida glabrata colonization in mice tissue.
Material and Method: The study was performed on 90 mature male mices (9 per group), that were devided into 10 groups: normal, placebo, 3 treatment group (candida- extract), control positive (Candida glabrata) and control negative (extract). Treatment groups were received 50,100 and 200 mg/kg dosages of extract for 20 days (every other day) by intraperitoneal injection. At the end of injections, homogenized liver, spleen and kidney were cultured on SDA medium and then colony count were performed.
Results: The results showed, that liver in 100 mg/kg, spleen and kidney in 200mg/kg haven’t any yeast.
Discussion and Conclusion: According to the results, Teucrium polium extract, perhaps can stimulat the immunity against Candida glabrata.

Keywords: Teucrium polium, Candida glabrata, liver, spleen, kidney

کلیدواژه‌ها [English]

  • Teucrium polium
  • Candida glabrata
  • Liver
  • spleen
  • kidney

مراجع

  1. Ubulom P, Akpabio E, Ejikeme Udobi C, Mbon R. Research In Pharmaceutical Biotechnology, 2011;3(5): 57- 60.
  2. Moussa A, Saad A, Djebli N.D, Meslem A, Benhalima E.K. International Journal Of Microbiological Research, 2011; 2(3): 276-279.
  3. Sarris J. Phytotherapy Research, 2007; 21: 703-716.
  4. Stefkov G, Kulevanova S, Miova B, Dinevska-Kjovkarovska S, Mølgaard P, Jäger A K, Josefsen K. Informa Healthcare, 2011; 49(2): 885-892.
  5. Zargari A. Iranian medicinal plants. Publishing and Printing Institute of Tehran University 1997.
  6.  Ghahreman A. Kuromophit of Plant Systematics, center of academic publishing printing. 1995; Volume 3.
  7. Khoocheki A, Azizi G. Effect of different treatments on breaking dormancy of Teucrium polium. Journal of agricultural research Iran 2006; 3(1): 81-88.
  1. Nadimi M, Zia M, Madani M. The Effect of Aqueous and Ethanolic Extracts of Teucrium polium on Candida albicans and two species of Malassezia, Zahedan Journal of Research in Medical Sciences  2013;15(8): 34-38.
  2. Heshmati S, Mahboobeh M, Amjad l. Study of Inhibitory Effect of Echinops cephalotes on Candida Spp. Isolated from Vulvovaginal Candidiasis Patients in Isfahan, Zahedan J Res Med Sci 2016 ; 18(6):e7355.
  3. Burket LW, Greenberg M, Glick M. Burket's oral medicine: diagnosis and treatment. Hamilton, Ont: BC Decker 2003.
  4. Koc M, Aktas E. Prophylactic treatment of mycotic mucositis in radiotherapy of patients with head and neck cancers. Jpn J Clin Oncol  2003; 33(2): 57-60.
  5. Perlroth J, Choi B, Spellberg B. Nosocomial fungal infections: epidemiology, diagnosis, and treatment. Med Mycol 2007; 45(4): 321-46.
  6. Ayatollahi Mousavi S A, Khalesi E, Shahidi Bonjar G H, Aghighi S, Sharifi F, Aram F. Rapid molecular diagnosis for Candida species using PCR-RFLP. Biotechnol 2007; 6: 583-587.
  7. Shoham S, Levitz SM. The immune response to fungal infections. Br J Haematol 2005; 129(5):569–82.
  8. Dongari-Bagtzoglou A, Dwivedi P, Ioannidou E, Shaqman M, Hull D, Burleson J. Oral Candida infection and colonization in solid organ transplant recipients. Oral Microbiol Immunol 2009; 24(3): 249-54.
  9. Fraser VJ, Jones M, Dunkel J, Storfer S, Medoff G, Dunagan WC. Candidemia in a tertiary care hospital: epidemiology, risk factors, and predictors of mortality. Clin Infect Dis 1992; 15(3): 414-21.
  10. Einloo A, Dehghan P, Saluti M, Mirzaahmadi S. Qualitative identification of Candida glabra by gold nanoparticle colorimetric method.Journal of Isfahan Medical School 2015: Vol. 33, No. 325, 1st Week.
  11. Stuyt RJ, Netea MG, van Krieken JH, van der Meer JW, Kullberg BJ. Recombinant interleukin-18 protects against disseminated Candida albicans infection in mice. J Infect Dis 2004; 189(8):1524–7.
  12. Alasvand Zarasvand M, Madani M, Modaresi M. The Effect of Hydroalcoholic Extract of Artemisia dracunculus L. (Tarragon) on Candida albicans Infection in Mice. Jundishapur J Nat Pharm Prod 2016; 11(4):e29911.
  13. Farahnejad Z, Ghazanfari T, Yaraee R. Immunomodulatory effects of Aloe vera and its fractions on response of macrophages against Candid albicans. Immunopharmacol immunotoxic 2011; 33(4):676–81.
  14. Darwish RM, Aburgia TA. Antimicrobial activity of some medicinal plants against different candida species. Jordan Journal of Pharmaceutical Science 2011; 1: 70-80.
  15. Hajyani S, Modaresi M, Madani M. Effect of Malva sylvestris L. extract on blood cell parameters in mice with Candida albicans Infection. Der Pharma Chemica 2015; 7(5):302-305.
  16. Bokaeian M, Eghbal ghorayshi M, Dabiri S. Antibiotic resistance of Neisseria gonorrhoeae strains isolated from gonorrhea. Zahedan Journal of Research in Medical Sciences 1389: Volume 12, Issue 2.
  17. Cushnie TP, Lamb Aj. antimicrobial activity of flavonoids, Intj Antimicrob Agents 2006; 27 (2): 181.
  18. Bokaeian M, Nakhaee A, Moodi B, Farhangi A, Akbarzadeh A. Effects of garlic extract treatment in normal and streptozotocin diabetic rats infected with Candida albicans. Indian J Clin Biochem 2010; 25(2):182.
  1. Elmasri W.A, Hegazy M-E.F, Aziz M, Koksal E, Amor W, Mechref Y, Hamood A.N, Cordes D.B, Pare P.W. Phytochemistry, 2014;103: 107-113.
  2. Baluchnejadmojarad T, Roghani M, Roghani-Dehkordi F. Journal Of  Ethno- Pharmacology, 2005; 97: 207-210.
  3. Soheili khah M, Salman roghani S, Mohammadi H. A case of hepatitis, cholestasis by drugs agronomic criteria. University of Medical Sciences and Health Services - Health yazd Sadoughi 2007; 15(3):9-97.
  4. Ziaee T, Razavi BM, Hosseinzadeh H. Saffron reduced toxic effects of its constituent, safranal, in acute and subacute toxicities in rats. JundishapurJNatPharmProd. 2014;9(1):3–8.
  5. Kesmati M, Izadi L, Mard-Soltani M. Effect of Matricaria recutita Hy- droalcoholicExtractonAnxietyBehaviorinMicebyHole-BoardTest. Zahedan JRes Med Sci. 2014;16(3):21–4.
  6. Petrovska BB. Historical review of medicinal plants’ usage. Phar- macogn Rev. 2012;6(11):1–5.
  7. Arbabi kalani F, Shirzaee M, Poor zamani M, Dabiri S. Journal of Research in Dental Sciences  2012; Volume 8, number 4, Title 9.
  8. Behzadi , Salehi Sirjani M, Madani M. In vitro inhibitory effects of Rhus Coriaria aqueous and alcoholic extracts on Candida Albicans. Complementary Medicine Journal 2015;  5(1).
  1. Shadzi SH. Medical Mycology Laboratory diagnostics and treatment methods. publications of SID unit esfahan 2009; print  11.
  2. Bineshian F, Yadegari MH, Sharifi Z, Akbari Eidgahi M, Nasr R. Identi- fication of Candida Species Using MP65 Gene and Evaluation of the CandidaalbicansMP65GeneExpressioninBALB/CMice.Jundishapur JMicrobiol. 2015;8(5
  1. Dhuley JN. Hamycin treatment of candidiasis in normal and dia- betic rats. FEMS Immunol Med Microbiol. 1999;26(2):175–80.
  1. Samsam shariat S. Extraction and extraction of effective substances of medicinal plants and its identification and evaluation methods. First edition, Manni Publishing, Isfahan, Iran 1371; 3-20.
  2. Samsam shariat S. Selection of Medicinal Plants, Second Edition, Manni Publishing, Isfahan, Iran 1386; 235.
  3. Mobasher M, Sasani P, Al davood J,Aramesh K, Larijani B. Reload moral guide working with laboratory animals. Iranian Journal of Medical Ethics and History, Special Issue on Research Ethics 2012; Volume 5.
  4. Bokaeian1 M, Iqbal-Qureshi M , Dabiri S. Antibiotic resistance of Neisseria gonorrhoeae isolated from gonorrhoeae patients. Journal of Research in Medical Sciences 2009; 12(2):18-23.
  5. Mobasher M, Nakhaei N, Sheibani V. Check the Ethical Treatment of Animals Research Laboratory qualitative methods. Journal of Ethics in Science and Technology, Special Issue on Medical Ethics 2007; the attachment 1.
  6. Shoaie N, Mohammadi P, Roudbar Mohammadi SH. Antifungal Effect of Teucrium polium and Zingiber officinale Extracts on Clinical isolates of Candida Species. Armaghane-danesh, Yasuj University of Medical Sciences Journal (YUMSJ) 2012; 17(5): 71.
  7. Eghtedar doost M, Barani R, Naseri M. Effect of MS14 on hematopoiesis and blood cells in Balb / c mice. Quarterly Journal of Medicinal Plants, Volume 11, Number 42.
  1. Naeini  A, Khosravi A, Tajbakhsh H, Yaraie R. Antidiabetic and Immunomodulatory Effects of Essential Oil and Extract of Anise in Laboratory Conditions. Scientific-Research Journal of Shahed University 1388, 82: 7-20.
  1. Doost mohamadi M. Evaluation of antibacterial effects of aqueous and ethanolic extracts of juice and nano particles on Staphylococcus epidermidis and Pseudomonas aeruginosa under laboratory and animal models. Journal of Microbiological Biotechnology 1391: Vol. 4, No. 14, pp. 29-38.
  1. Bonyadpour B, Akbarzdeh M, Pakshir K, Mohagheghzadeh A. In vitro Susceptibility of Fluconazole, Clotrimazole and Toucrium Polium Smoke Product on Candida Isolates of Vaginal Candidiasis. Armaghane Journal 2009; 14(2):87-96.
  2. Khan MAV, Zuberi NS, Ashfag MK, Mahmood MS and Gilani AH. 2003. The in vivo antifungal activity of the aqueous extract from Nigella sativa seeds. Phytother. Res. 17: 183 - 6.
  3. Salbi M, Madani M, Modaresi M. Effects of Teucrium polium aquatic extract on blood cell parameters of Candida albicans infected in mice. Journal of Chemical and Pharmaceutical Research 2015; 7(5):398-401.

 

 

مجله علمی - پژوهشی دانشگاه علوم پزشکی سبزوار
دوره 25، شماره 1
فروردین و اردیبهشت 1397
صفحه 151-160

فایل ها

سابقه مقاله

  • تاریخ دریافت: 06 اردیبهشت 1396
  • تاریخ بازنگری: 19 مرداد 1396
  • تاریخ پذیرش: 09 خرداد 1397
  • تاریخ اولین انتشار: 09 خرداد 1397

اشتراک گذاری

ارجاع به این مقاله

آمار