بررسی فعالیت‌های ضدمیکروبی و آنتی‌اکسیدانی و ترکیبات فیتوشیمیایی اسانس و عصاره‌های مختلف گیاه کاه‌مکی (.Cymbopogon olivieri (Boiss.) Bor) از استان سیستان و بلوچستان

نوع مقاله : مقاله پژوهشی

نویسندگان

1 مرکز پژوهشی گیاهان دارویی و زینتی دانشگاه سیستان و بلوچستان

2 آزمایشگاه باکتریولوژی، شرکت آب و فاضلاب روستایی سیستان و بلوچستان، زاهدان، ایران

3 استادیار، گروه گیاهان دارویی، دانشکده کشاورزی، دانشگاه سیستان و بلوچستان، زاهدان، ایران

4 آزمایشگاه مرکزی، دانشگاه سیستان و بلوچستان، زاهدان، ایران

چکیده

اهداف: در این پژوهش فعالیت‌های ضدمیکروبی، آنتی‌اکسیدانی و ترکیبات فیتوشیمیایی اسانس و عصاره‌های گیاه دارویی کاه‌مکی (.Cymbopogon olivieri (Boiss.) Bor)، رشد‌یافته در استان سیستان و بلوچستان بررسی شده است.
مواد و روش‌ها: جهت بررسی ترکیبات فیتوشیمیایی شامل ترکیبات فنولی و فلاونوئیدی کل موجود در سه عصاره (متانولی، اتانولی و آبی) و اسانس، به ترتیب، روش‌های معرف فولین-سیوکالتیو، روش رنگ سنجی کلرید آلومینیوم و GC-MS و فعالیت آنتی‌اکسیدانی عصاره‌های مختلف به دو روش DPPH و FRAP و فعالیت ضدمیکروبی ترکیبات موجود در اسانس و عصاره‌ها علیه سه باکتری استافیلوکوکوس اورئوس، باسیلوس سرئوس، اشرشیاکلی و دو قارچ آسپرژیلوس نایجر و کاندیدا آلبیکنس به دو روش انتشار از چاهک و حداقل غلظت مهارکننده رشد میکروب (MIC) استفاده شد.
یافته‌ها: نتایج نشان دادند که عصاره متانولی دارای بیشترین میزان ترکیبات فنولی و فلاونوئیدی و فعالیت آنتی‌اکسیدانی و در مقابل، عصاره آبی دارای حداقل میزان این ترکیبات و فعالیت بوده است. در بررسی ترکیبات موجود در اسانس، سه ترکیب پیپریتون (95/82%) ، کارن (493/2%) و لیمونن (086/1%) از عمده‌ترین ترکیبات بودند. بررسی نتایج حاصل از اثرات ضدمیکروبی نشان دادند که اسانس و عصاره‌ها اثر مهارکنندگی قابل ملاحظه‌ای بر روی همه‌ی باکتری‌ها و قارچ‌های مورد استفاده نشان دادند بطوریکه این اثر کاملا وابسته به غلظت بوده‌است.
نتیجه‌گیری: بطورکلی این مطالعه نشان داد که این گیاه می‌تواند کاندیدای خوبی جهت درمان بیماری‌های ناشی از استرس‌های اکسیداتیوی و بیماری‌های ناشی از میکروب‌های بیماری‌زا قرار گیرد. همچنین با توجه به قدرت بالای این گیاه در ازبین بردن عوامل بیماری‌زا، می‌تواند جایگزین مناسب برای آنتی بیوتیک‌ها باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Investigation of antioxidant and antimicrobial activities and phytochemical Compounds of essential oil and different extracts of Cymbopogon olivieri (Boiss.) Bor. from Sistan and Baluchestan province

نویسندگان [English]

  • Omid Azizian Shermeh 1
  • Mozhgan Taherizadeh 2
  • Moharam Valizadeh 3
  • Alireza Zaboli 4
1 Medicinal and Ornamental Plant Research Center, University of Sistan and Baluchestan
2 Bacteriological Laboratory, Central Laboratory, Rural Water and Sewage Company of Sistan and Baluchestan, Zahedan, Iran
3 Assistant Professor, Department of Medicinal Plants, Faculty of Agriculture, University of Sistan and Baluchestan, Zahedan, Iran
4 Central Laboratory, University of Sistan and Baluchestan, Zahedan, Iran
چکیده [English]

Background: In this study antimicrobial activities, antioxidant activities and phytochemical compounds in the essential oil and extracts of Cumbopogon olivieri were studied.
Material & Methods: For investigation of phytochemical compounds such as total phenolic and total flavonoid contents in three extracts (Methanolic, ethanolic and aqueous) and essential oil by Folin-Ciocaltiu, Aluminum Chloride colorimetric and GC-MS methods and antioxidant activities of extracts by two methods (DPPH and FRAP) and antimicrobial activities of essential oil and extracts of Cumbopogon olivieri against three bacteria such as Staphylococcus aureus, Bacillus cereus, Escherichia coli and two fungi such as Candida albicans and Aspergillus niger by two methods (well diffusion and Minimal Inhibitory Concentration (MIC)) respectively.
Results: The results showed that, the methanolic extract had maximum total phenolic and flavonoid contents and antioxidant activity and aqueous extract had minimum value of this compounds and activity. In investigation of essential oil, three compounds such as piperitone (82.95%), carene (2.493%) and limonene were the major constituents. The results of antimicrobial activity showed that, the essential oil and extracts had good inhibition effect on all of bacteria and fungi and this inhibition depends to concentration.
Conclusion: Overall, based on the results obtained, the plant can be a good candidate for the treatment of diseases caused by oxidative stress, and diseases caused by pathogenic microbes. Also due to power up the plant in the elimination of pathogens, could be a viable alternative to antibiotics.

کلیدواژه‌ها [English]

  • Essential oil
  • Phytochemical Compounds
  • Antioxidant activity
  • Antimicrobial activity
  • Cymbopogon olivieri (Boiss.)
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دوره 25، شماره 2
خرداد و تیر 1397
صفحه 279-292
  • تاریخ دریافت: 16 اسفند 1395
  • تاریخ بازنگری: 21 تیر 1396
  • تاریخ پذیرش: 21 مرداد 1396
  • تاریخ اولین انتشار: 01 خرداد 1397