بررسی مقایسه ای سمیت نانوذره نیکل و کلرید نیکل بر بافت و آنزیم های کبدی در موش صحرایی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناس ارشد بیوشیمی-گروه زیست سلولی و مولکولی، دانشکده علوم زیستی، دانشگاه آزاد اسلامی واحد تهران شمال، تهران، ایران

2 گروه زیست سلولی و مولکولی-دانشکده علوم پایه-بابلسر-مازندران-ایران

3 گروه زیست سلولی و مولکولی، دانشکده علوم پایه، دانشگاه مازندران، بابلسر، ایران

4 گروه زیست سلولی و مولکولی، دانشکده علوم زیستی، دانشگاه آزاد اسلامی واحد تهران شمال، تهران، ایران

چکیده

اهداف: سمیت فلزات و ترکیبات آن توسط خواص فیزیکوشیمیایی تعیین می شود. بنابراین، فلزات ممکن است برای اتصال به سایت های بیولوژیکی به رقابت بپردازند و در نتیجه باعث عملکرد نادرست ماکرومولکول های بیوشیمیایی شوند. این مطالعه با هدف بررسی سمیت نانوذره نیکل در مقایسه با کلرید نیکل بر فعالیت آنزیم های کبدی و بررسی تغییرات هیستوپاتولوژی بافت کبد در موش صحرایی نر انجام شد.
مواد و روش ها: در این مطالعه تجربی، 48 سر موش صحرایی نر بالغ به 6 گروه تجربی، یک گروه کنترل ویک گروه شم تقسیم شدند. گروه های تجربی، نانوذرات نیکل و کلرید نیکل با غلظت 5 ، 15 و 25 میلی گرم بر کیلوگرم به صورت درون صفاقی دریافت کردند. پس از یک هفته تزریق، خون و نمونه بافتی جهت آنالیز جمع آوری شد. سنجش فعالیت آنزیم های کبدی و مطالعات هیستوپاتولوژی انجام شد. نتایج با استفاده از آنوا وتست تعقیبی توکی آنالیز شد.
یافته ها: فعالیت آنزیم های ASTوALP در دوزهای مختلف نانوذره نیکل و کلرید نیکل و فعالیت LDH در دوزهای مختلف کلرید نیکل تغییرات معنی داری در گروه های مورد مطالعه نشان داد (05/0p

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Comparative study on toxicity of Ni nanoparticle and Nickel chloride on liver enzymes and tissue in Rats

نویسندگان [English]

  • Farnosh Anoosha 1
  • Bagher Seyedalipour 2
  • Ali Taravati 3
  • Monireh Movahedi 4
1 Department of Cellular and Molecular Biology, Faculty of Life Sciences, Islamic Azad University, Tehran North Branch, Tehran, Iran.
2 Department of Cellular and Molecular Biology, faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
3 Department of Cellular and Molecular Biology, faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
4 Department of Cellular and Molecular Biology, Faculty of Life Sciences, Islamic Azad University, Tehran North Branch, Tehran, Iran.
چکیده [English]

Background & Objectives: The toxicity of metals and their compounds is determined by their physicochemical properties. Thus, metals may compete for the biological binding sites and lead to incorrect function of biochemical macromolecules. The aim of this study was to investigate Ni NPs toxicity in compared to Nickel chloride on liver enzyme activity and the histopathological changes of liver tissue in male wistar rats.
Materials and Methods: In this experimental study, 48 male rates were divided into six experimental groups and one control group and one sham group (n=6). Experimental groups received Ni NPs and nickel chloride with concentration of 5, 15 and 25 mg/ kg by intraperitoneal injection. At the end of 7th day, blood and liver specimen was collected for analysis. Assay of the activity of liver enzyme and histopathological study were performed. Data were analyzed using ANOVA and Tukey test.
Results: Enzyme activities of AST and ALP in different doses of Ni NPs and nickel chloride and LDH activity in different doses of nickel chloride showed significant changes in the studied groups (p > 0.05). Histopathological study of liver were revealed dose dependent changes in rats treated with nickel chloride and nanoparticles compared to the control group. Some of the changes including necrosis, congestion, apoptosis and steatohepatitis was observed after exposure to different doses.
Conclusion: Increasing of concentration liver enzymes and histopathological changes confirms the toxicity of Ni NPs and nickel chloride

کلیدواژه‌ها [English]

  • Nickel nanoparticles
  • Nickel chloride
  • liver enzymes
  • Rat
  • Histopathological
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