1دانشجوی دکتری، گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه آزاد اسلامی واحد تهران مرکزی، تهران، ایران
2گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه آزاد اسلامی واحد تهران مرکزی، تهران، ایران
3دانشیار گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه آزاد اسلامی واحد تهران مرکزی، تهران، ایران
تاریخ دریافت: 23 آذر 1397،
تاریخ بازنگری: 08 بهمن 1397،
تاریخ پذیرش: 23 تیر 1398
چکیده
چکیده زمینه: امروزه استفاده از مکمل‌ها و عصاره‌های گیاهی مانند مرزنجوش برای مقابله با آپوپتوز ناشی از ورزش و بهبود سازگارهای‌های تمرینی رواج بسیاری یافته است. بنابراین، مطالعه حاضر با هدف تعیین تأثیر همزمان تمرین هوازی و مکمل‌سازی عصاره گیاهی مرزنجوش بر بیان ژنی پروتئین‌های آپوپتوز میوکارد موش‌های صحرایی انجام شد. روش کار: در این مطالعه تجربی، 40 سر موش صحرایی نر نژاد ویستار (8 هفته‌ای) با میانگین وزنی (86/17± 129 گرم) به صورت تصادفی در پنج گروه همگن، پایه (8n= )، کنترل(8n= )، عصاره(8n= )، تمرین(8n= ) و تمرین - عصاره (8n= ) قرار گرفتند. گروه تمرین و تمرین-عصاره در یک برنامه 12 هفته‌ای(هر هفته 5 جلسه‌ی 60-10 دقیقه‌ای با شدت80-75 درصد اکسیژن مصرفی بیشینه) دویدن روی نوارگردان حیوانی(شیب 15درصد و سرعت 33-24 متر بر دقیقه) شرکت کردند. همچنین، مکمل‌سازی مرزنجوش شامل دریافت عصاره اتانولی مزرنجوش بود. 48 ساعت پس از آخرین جلسه تمرینی، بخشی از بافت بطن چپ قلب موش‌ها برداشته شد و بیان پروتئین‌هایBax، Bcl-2 و کاسپاز-9 با استفاده از روش RT-PCR بررسی شد. داده‌ها، با استفاده از آزمون تحلیل واریانس دوراهه تجزیه و تحلیل شد(P
The effect of concurrent aerobic training with Origanum vulgare Hydro Ethanolic Extract supplementation on gene expression of myocardial apoptosis proteins in male rats
1PhD Student at Islamic Azad University, Central Tehran Branch, Department of Sport Sciences, Faculty of Physical Education and sport sciences, Tehran, Iran
2Professor at Islamic Azad University, Central Tehran Branch, Department of Sport Sciences, Faculty of Physical Education and sport sciences, Tehran, Iran
3Associate Professor at Islamic Azad University, Central Tehran Branch, Department of Sport Sciences, Faculty of Physical Education and sport sciences, Tehran, Iran
چکیده [English]
Abstract Background: Today, the use of supplements and herbal extracts has been widely used to prevent exercise induced apoptosis and improve exercise training adaptations. Thus, the present study examined the effect of the 12 weeks aerobic training with Origanum vulgare ethanolic Extract supplementation on the myocardial apoptosis in young male rats. Methods: In this experimental study,40 male Wistar rats (8 weeks old)with an average(weight 129±17.86 in gr)randomly divided in five equal groups:basic(n=8),control(n=8),Origanum vulgare(n=8), aerobic training(n=8)and aerobic training-Origanum vulgare(n=8).The aerobic training and aerobic training - Origanum vulgare groups participated in a 12-week program (5 sessions of 10-60 minutes each week, with an intensity of 75-80% of maximum oxygen consumption) running on animal treadmill (15% incline and 24-33 m/s).Also,Origanum vulgare Ethanolic extract was used for supplementation.Forty-eight hours after the last exercise session,a part of the left venticular tissue of the heart was removed and expression of Bax,Bcl2 and caspase-9 proteins was investigated using RT-PCR method. Data were analyzed byTwo-way ANOVA test (P
کلیدواژه‌ها [English]
Aerobic Exercise, Origanum vulgare, apoptosis, Rat
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