بررسی فعالیت ضد قارچی باکتری های بومی جدا شده از خاک کشاورزی خرم آباد

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه زیست شناسی، دانشکده علوم پایه؛ دانشگاه آزاد اسلامی واحد بروجرد، ایران

2 گروه زیست شناسی، دانشکده علوم پایه، دانشگاه علوم، دانشگاه لرستان، خرم آباد، ایران

3 پژوهشکده بیوتکنولوژی کشاورزی، کرج، ایران

4 گروه زیست شناسی، دانشکده علوم پایه؛ دانشگاه پیام نور، تهران، ایران

5 گروه زیست شناسی، دانشکده علوم پایه؛ دانشگاه لرستان، ایران

چکیده

زمینه و هدف: ظهور گونه‌های مقاوم به داروها و همچنین محدودیت استفاده از داروهای ضد قارچی به دلیل اثرات سمی احتمالی در انسان، هزینه بالای تولید آن‌ها و آلودگی محیط‌زیست ناشی از مصرف این داروها تحقیقات موردنیاز برای یافتن ترکیبات ضد قارچی جدید را ضروری دانسته است. میکروارگانیسم‌ها به‌ویژه باکتری‌های خاک طیف گسترده‌ای از مواد ضد میکروبی و فعال را تولید می‌کنند که برخی از آن‌ها خاصیت ضد قارچی دارند. هدف از این مطالعه جداسازی باکتری‌های مؤثر بر رشد قارچ‌ها، از خاک کشاورزی شهرستان خرم‌آباد است.
مواد و روش‌ها: نمونه‌برداری از خاک کشاورزی مناطق مختلف شهرستان انجام شد. سپس جداسازی باکتری‌های خاک با استفاده از محیط نوترینت براث و نوترینت آگار انجام و به دنبال آن اثر ضد قارچی مایع رویی و همچنین عصاره آن‌ها به روش انتشار دیسک در محیط SCA بر روی چندین گونه قارچ مورد بررسی قرار گرفت.
یافته‌ها: در کل چهار جدایه، دارای اثرات ضد قارچی قابل‌ملاحظه‌ای بر روی برخی قارچ‌ها شامل Aspergillus niger،Aspergillus flavus،Aspergillus fumigatus و Candida albicans بودند. این جدایه‌ها با استفاده از آزمون‌های بیوشیمیایی و تعیین توالی ژن SrRNA16 شناسایی شدند. پس از جستجوی توالی آن‌ها از طریق BLAST در بانک اطلاعاتی NCBI جنس باکتری‌ها متعلق به دو جنس Pseudomonas و Acinetobacter بودند.
نتیجه‌گیری: این اولین گزارش از بررسی فعالیت ضد قارچی، باکتری‌های جداشده از خاک کشاورزی خرم‌آباد است. این باکتری‌ها می‌توانند کاندید مناسبی برای کنترل زیستی قارچ‌های بیماری‌زا باشند. استخراج، شناسایی و به‌کارگیری متابولیت‌های مهاری آن‌ها می‌تواند در طراحی و تولید داروهای ضد قارچی استفاده شود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Antifungal activity of native bacteria isolated from Khorramabad agricultural soil

نویسندگان [English]

  • Amir Hadi Chegeni 1
  • Faranak Hadi 2
  • Mojgan kowsari3 3
  • Maryam Zare 4
  • Abdolnaser Gholami Mohammadi 5
1 Department of Biology, Faculty of science, Azad University, Brojerd, Iran
2 Biology department, Faculty of science, Lorestan university, Khorramabad, Iran
3 Agricultural Biotechnology Research Institute of Iran, Karaj, Iran
4 Department of Biology, Faculty of Science, Payamme Noor University, Tehran, Iran
5 Department of Biology, Faculty of Science, Lorestan University, Khorramabad, Iran
چکیده [English]

Background: Emergence of drug resistant strains and the limited use of antifungal drug due to possible toxic effects on humans, the high cost of their production and environmental pollution has made the search for novel bio-antifungal compounds a necessity. Microorganisms, particularly soil bacteria produce a wide range of active antimicrobial substances that some of them have antifungal properties. The purpose of this study is isolation and identification of the bacteria affecting the growth of fungi from Khorramabad agricultural soil.
Materials and Methods: Agricultural soils were collected from different areas of Khorramabad, Lorestan Province. The bacteria were isolated using nutrient broth and nutrient agar, then, the antifungal activity of their extracts and supernatant in the SCA media on several species of fungi was studied applying disk diffusion method.
Results: Four strains showing a remarkable antifungal effect against some fungi, including Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus and Candida albicans. These isolates were identified by biochemical features and 16S rRNA sequence. Analysis of their sequencing through BLAST in NCBI data bank showed that these bacterial similarly closed to Pseudomonas sp. and Acinetobacter sp. genus.
Conclusion: This study was the first report of antifungal analysis of soil isolated bacteria from Khorramabad. These bacteria could be a potential candidate for biocontrolling pathogenic fungi.The extraction, identification and development of their inhibitory metabolites can be used in the design and production of antifungal agents.

کلیدواژه‌ها [English]

  • Bacterial isolation
  • Antifungal
  • Disk diffusion method
  • 16SrRNA
 
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دوره 26، شماره 3
مرداد و شهریور 1398
صفحه 383-391
  • تاریخ دریافت: 06 فروردین 1397
  • تاریخ بازنگری: 22 مهر 1397
  • تاریخ پذیرش: 30 شهریور 1398
  • تاریخ اولین انتشار: 30 شهریور 1398