بررسی و مقایسه فعالیت زیستی اسانس Mentha longifolia از مریوان و قزاآن

نوع مقاله : مقاله پژوهشی

نویسندگان

1 فیتوشیمی، گروه فیتوشیمی، دانشکده شیمی، دانشگاه کاشان، کاشان، ایران

2 میکروبیولوژی، گروه بیوتکنولوژی، دانشکده شیمی، دانشگاه کاشان، کاشان، ایران

چکیده

زمینه و اهداف: از آنجا که آنتی‌اکسیدان‌ها، آنتی‌بیوتیک‌ها و مواد ضدسرطان مصنوعی دارای اثرات نامطلوب بر سلامتی انسان هستند، در این پژوهش فعالیت‌های زیستی اسانس‌های پونه مریوان و قزاآن بررسی و با یکدیگر مقایسه شدند تا جایگزین طبیعی مناسبی برای ترکیبات مصنوعی ذکر شده، شناسایی گردد.
مواد و روش ها: اسانس برگ‌ها و ساقه‌های پونه مریوان و قزاآن با امواج میکرویو و بدون استفاده از حلال استخراج شد. ترکیبات تشکیل دهنده اسانس‌ها با دستگاه کروماتوگرافی گازی-طیف‌سنج جرمی شناسایی شدند. فعالیت آنتی‌اکسیدانی اسانس‌ها با روش بی‌رنگ شدن بتاکاروتن، سمیت سلولی آن‌ها با آزمون کشندگی میگوی آب شور و فعالیت ضدمیکروبی آن‌ها با روش انتشار در آگار و تعیین کمترین غلظت مهارکننده رشد بررسی گردید.
یافته ها: ترکیب اصلی در اسانس پونه مریوان پولگون (%45/81) و ایزوپولگول (%39/8) و در اسانس پونه قزاآن پولگون (%29/48) و پیپریتنون اکسید (%53/23) بودند. درصد بازدارندگی اسانس پونه مریوان و قزاآن و استاندارد BHT به ترتیب %02/80، %59/78 و %50/95 و مقدار LC50 اسانس پونه مریوان و قزاآن و داروی ضدسرطان وین‌کریستین‌سولفات به ترتیب 47/53، 96/55 و 751/0 میکروگرم بر میلی‌لیتر به دست آمد. اسانس‌های پونه مریوان و قزاآن فعالیت ضدمیکروبی خوبی به خصوص برعلیه باکتری‌های گرم مثبت نشان دادند.
نتیجه‌گیری: اسانس‌های پونه مریوان و قزاآن دارای فعالیت آنتی‌اکسیدانی، ضدمیکروبی و ضدسرطانی خوبی هستند و می‌توان آن‌ها را به عنوان جایگزینی برای آنتی‌اکسیدان‌ها، آنتی‌بیوتیک‌ها و مواد ضدسرطان مصنوعی معرفی نمود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Comparative evaluation of essential oil bioactivities of Mentha longifolia from Marivan and Qaza An

نویسندگان [English]

  • Morteza Yazdani 1
  • Fereshteh Jookar Kashi 2
  • Akram Rahimi-Moghaddam 2
1 Department of Phytochemistry, Faculty of Chemistry, university of Kashan, Kashan, Iran
2 Department of Biotechnology, Faculty of Chemistry, university of Kashan, Kashan,
چکیده [English]

Background: Considering the adverse effects of synthetic antioxidants, anticancer drugs and antibiotics on human health, in this study, bioactivities of Mentha longifolia essential oils collected from Marivan and Qaza An were examined and compared with each other for identification a natural alternative of synthetic antioxidants, anticancer drugs and antibiotics.
Materials and Methods: Essential oil of M. longifolia leaves and stems collected from Marivan and Qaza An was prepared using solvent-free microwave extraction method. The essential oil components were identified by gas chromatography/mass spectrometry. The antioxidant and cytotoxicity activity of the essential oils were determined via β–carotene bleaching assay and brine shrimp lethality test, respectively. The antimicrobial activity of the essential oils was evaluated by the agar well diffusion method and by determination of minimum inhibitory concentration.
Results: The main components of M. longifolia essential oil collected from Marivan were pulegone (81.45%) and isopulegol (8.39%) and the main components of M. longifolia essential oil collected from Qaza An were pulegone (48.29%) and piperitenone oxide (23.53%). The inhibition of linoleic acid oxidation by M. longifolia essential oils collected from Marivan, Qaza An and BHT were 80.02%, 78.59% and 95.50%, respectively. The LC50 of the M. longifolia essential oils collected from Marivan, Qaza An and vincristine sulfate were 53.47μg/ml, 55.96 μg/ml and 0.751 μg/ml, respectively. M. longifolia essential oils had good antimicrobial activity, especially against gram-positive bacteria.
Conclusion: M. longifolia essential oils have significant antioxidant, antimicrobial and anticancer activity and can be introduced as alternatives of synthetic antioxidants, antibiotics and anti-cancer drugs.

کلیدواژه‌ها [English]

  • Mentha longifolia
  • Essential oil
  • Antimicrobial activity
  • Antioxidant activity
  • Cytotoxicity
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