بررسی اثر سمیت سلولی سه ترکیب هومیوپاتی سپیا، فسفر و پولستیلا بر سلول‌های سرطانی پستان MCF-7

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشیار، مرکز تحقیقات سلولی و مولکولی، گروه فیزیولوژی و فارماکولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی سبزوار، سبزوار، ایران

2 کارشناس ارشد بیولوژی سلولی، مرکز تحقیقات سلولی مولکولی، دانشگاه علوم پزشکی سبزوار، سبزوار، ایران

3 دکتری داروسازی، دانشکده داروسازی، دانشگاه علوم پزشکی مشهد، مشهد، ایران

4 استادیار، مرکز تحقیقات دارویی، دانشکده داروسازی، دانشگاه علوم پزشکی مشهد، مشهد، ایران

چکیده

زمینه و هدف  سرطان پستان، دومین سرطان رایج در زنان سراسر جهان می‌باشد. علاوه بر این، بیماران مبتلا به این سرطان، عموماً از طب مکمل و مداخله‌گر همچون هومیوپاتی استفاده می‌کنند. مشخص شده است که ترکیبات هومیوپاتی از قبیل سپیا (Sepia)، فسفر(Phosphorus)  و پولستیلا (Pulsatilla) تأثیرات شایانی در انواع بیماری‌ها از قبیل برخی سرطان‌ها دارند. به هرحال، تاکنون، سمیت سلولی این ترکیبات در محیط برون‌تن بر رده سلولی سرطان پستان نظیر MCF-7 مطالعه نشده است. از این رو، هدف این مطالعه، بررسی تأثیر سمیت این ترکیبات هومیوپاتیک بر روی رده سلولی سرطان پستان می‌باشد.
مواد و روش‌ها سلول‌های سرطانی پستان (MCF-7) در محیط کشت DMEM کشت گردیدند و سپس با ترکیبات هومیوپاتیک با پوتنسی‌های 30 و 200 به مدت 24، 48 و 72 ساعت تیمار گردیدند. سپس پایایی سلول‌ها از طریق سنجش MTT بررسی شدند.
یافته‌ها یافته‌ها بیان می‌کنند که هیچ‌کدام از ترکیبات آزمون شده بر سلول‌های سرطانی MCF-7 دارای تأثیر سمیت سلولی در محیط برون‌تن نمی‌باشند.
نتیجه‌گیری این مطالعه نشان داد که سپیا، فسفر و پولستیلا به‌عنوان سه ترکیب شاخص هومیوپاتی فاقد اثر سمیت سلولی بر رده سلولی سرطانی پستان بود و به نظر می‌رسد که مطالعات بیشتری بر روی سایر سلول‌ها و نیز مطالعات درون‌تن برای ارزیابی بیشتر موردنیاز می‌باشد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Evaluation of cytotoxic effects of three homeopathy remedies (Sepia, Phosphorus and Pulsatilla) in the breast cancer cell line, MCF-7

نویسندگان [English]

  • bahareh amin 1
  • LEILA aldaghi 2
  • hamideh moalemzadeh 3
  • fatemeh soltani 4
1 Associate Professor, Cellular and Molecular Research Center, Department of Physiology and Pharmacology, Sabzevar University of Medical Sciences, Sabzevar, Iran
2 MSC cell biology, Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
3 Dr in Pharmacy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
4 Assistant Professor, Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده [English]

Introduction: Breast cancer is the second common cancer in women at worldwide. Furthermore, patients with breast cancer generally use complementary  and integrative medicine such as homeopathy. It has been shown that homeopathic remedies such as Sepia, Phosphorus and Pulsatilla have great effects in the various diseases such as some of the cancers. However, the cytotoxicity effects of these compounds have not been studied for breast cancer cell lines such asMCF-7. Hence, the aim of this study was to investigate the cytotoxic effects of these homeopathic remedies on breast cancer cell line.
Materials & Methods: Breast cancer cells (MCF-7) were cultured in DMEM medium and treated with different potencies (30 and 200) of remedies for 24, 48 and 72 h. Cell viability was determined by MTT assay.
Results: The findings indicate that none of the tested compounds had cytotoxicity effect on MCF-7 cancer cells.
Conclusion: This study shows that Sepia, Phosphorus and Pulsatilla as the three of homeopathic remedies have not in vitro cytotoxic effects on breast cancer cell line, and it seems that further studies on different cell lines and also in vivo studies are needed to confirm these findings. 

کلیدواژه‌ها [English]

  • Homeopathy
  • Breast Cancer
  • Sepia
  • Phosphorus
  • Pulsatilla
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دوره 28، شماره 2
خرداد و تیر 1400
صفحه 207-213
  • تاریخ دریافت: 14 مهر 1398
  • تاریخ بازنگری: 02 بهمن 1398
  • تاریخ پذیرش: 02 بهمن 1398
  • تاریخ اولین انتشار: 01 خرداد 1400