بررسی تأثیر تنش خشکی (پلی‌اتیلن گلیکول) بر صفات آنتی‌اکسیدانی و برخی صفات فیزیولوژیکی گیاه دارویی پنیرباد (Withania coagulans Dunal)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکترای تخصصی (باغبانی) گرایش گیاهان دارویی، دانشگاه آزاد اسلامی واحد شیروان، شیروان، ایران

2 دانشیار بخش تحقیقات علوم زراعی و باغی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی خراسان رضوی، سازمان تحقیقات، آموزش و ترویج کشاورزی، ایران

3 استادیار گروه زراعت، دانشکده کشاورزی، دانشگاه آزاد اسلامی واحد شیروان، شیروان، ایران

4 استادیار گروه مهندسی شیمی، دانشگاه آزاد اسلامی واحد قوچان. قوچان، ایران

چکیده

زمینه و هدف  گیاه دارویی پنیرباد با دارا بودن اثرات ضدقارچی و ضدباکتریایی، کاهش‌دهنده قند و کلسترول خون، تقویت‌کننده سیستم ایمنی و آنتی‌اکسیدانی اخیراً موردتوجه فارماکولوژیست‌ها قرار گرفته است و به دلیل سازگاری بسیار وسیع نسبت به شرایط دشوار اکولوژیکی می‌تواند در مناطق گرمسیر کشور از جمله استان سیستان‌وبلوچستان رشد کند.
مواد و روش‌ها این مطالعه، تحت شرایط آزمایشگاهی در مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان خراسان رضوی در سال 139۷ در قالب طرح کاملاً تصادفی با چهار تکرار طراحی شد. غلظت‌های صفر، 2، 4، 6، 8 و 10 بار پلی‌اتیلن‌گلایکول برای القای تنش خشکی در محیط کشت MS حاوی بنزیل‌آدنین‌پورین بر صفات ثانویه گیاه پنیرباد مورد استفاده قرار گرفت.
یافته‌ها نتایج نشان داد که تنش خشکی 10 بار منجر به افزایش معنی‌دار فعالیت آنزیم‌های آنتی‌اکسیدان کاتالاز، پراکسیداز و آسکوربات پراکسیداز، محتوی پرولین، فنل و فلاونوئید و میزان نشتی غشا شد ولی از طرف دیگر، منجر به کاهش معنی‌دار محتوای رطوبت نسبی، محتوی یون پتاسیم و نسبت یون پتاسیم به سدیم گردید. همچنین بیشتر صفات موردمطالعه، همبستگی معنی‌دار دارند.
نتیجه‌گیری نتایج نشان داد که گیاه دارویی پنیرباد از تحمل قابل‌قبولی در برابر القای تنش خشکی با پلی‌اتیلن‌گلایکول برخوردار است. در کاهش اثرات منفی ناشی از تنش خشکی مکانیسم‌های آنزیمی (فعالیت آنزیم‌های آنتی‌اکسیدانت) و غیرآنزیمی (محتوی پرولین، فنل، فلاونوئید و غیره) نقش بسیار مهمی دارد. از طرف دیگر، تنش خشکی، منجر به افزایش برخی متابولیت‌ها همچون فنل و فلاونوئید که مصارف دارویی دارند، شد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of drought stress (polyethylene glycol) on antioxidant and some physiological traits of Paneer-boot (Withania coagulans Dunal)

نویسندگان [English]

  • Aysan Ghahremani 1
  • Ebrahim Ganji_Moghadam 2
  • Maryam Tatari 3
  • Susan Khosroyar 4
1 Ph.D. student, Department of Horticultural Sciences, Shirvan Branch, Islamic Azad University, Shirvan, Iran
2 Associate Professor, Crop And Horticultural Science Research Department, Khorasan Agricultural and Natural Resources Research and Education Center, Areeo Mashhad, Iran
3 Assistant Professor, Department of Agronomy, Shirvan Branch, Islamic Azad University, Shirvan, Iran.
4 Assistant Professor, Department of Chemical Engineering, Quchan Branch, Islamic Azad University, Quchan, Iran
چکیده [English]

Introduction: Paneer-boot, with its antifungal and antibacterial effects, blood sugar and cholesterol-lowering, immune system, and antioxidant activity, has recently received pharmacologists' attention due to its high compatibility. Due to difficult ecological conditions, it can grow in the country's tropical regions such as Sistan and Baluchestan Province .
Materials & Methods: This study was designed in a completely randomized design with four replications under laboratory conditions at Khorasan Razavi Agricultural and Natural Resources Research and Education Center. Concentrations of 0, 2, 4, 6, 8, and 10 bar PEG were used to induce the effect of drought stress on MS medium containing BAP on secondary traits of cheese .
Results: The results showed that the drought stress significantly increased antioxidant enzymes such as catalase, peroxidase, ascorbate peroxidase, proline, phenol, and flavonoid content membrane leakage. On the other hand, it resulted in a significant decrease in the relative humidity content, potassium ion content and potassium to sodium ratio. Also, most of the studied traits had a significant correlation. Conclusion: The results showed that the Paneer-boot plant had acceptable tolerance to induction of drought stress with polyethylene glycol. Enzymatic and non-enzymatic mechanisms had a significant role in reducing the adverse effects of drought stress. On the other hand, drought stress increased some metabolites, such as phenol and flavonoids, which have medicinal uses .

کلیدواژه‌ها [English]

  • Proline
  • Paneer-boot
  • Antifungal
  • Flavonoid
  • Catalase
[1]. Valizadeh M. Evaluation of different masses of chewable medicinal herbs and in vitro production and anti-cancer potential of VITAFRINE A: Ferdowsi University of Mashhad; 2015;1:1-5.
[2]. Kaykha Z, Valizadeh M, Valizadeh J, Taheri KH. Studying the quantity and quality of fatty acids in the seeds of Withania coagulans (Stocks) Dun. and Withania somnifera (L.) Dun. collected from different habitats of Sistan and Baluchestan. Iranian Journal of Medicinal and Aromatic Plants. 2012; 33:730-740. [3]. Ghahremani A, Ganji-Moghadam E, Tatari M, Khosroyar S. Physiological responses of paneer-booti (Withania coagulans Dunal) to nacl stress under tissue culture conditions. Notulae Botanicae Horti Agrobotanici ClujNapoca. 2019; 47(4):1365-1373.
[4]. Kazemipour N, Salehi Inchebron M, Valizadeh J, Sepehrimanesh M. Clotting characteristics of milk by Withania coagulans: Proteomic and biochemical study. International Journal of Food Properties. 2017; 20(6):1290301.
[5]. Esmaeilzadeh Behabadi S, Sharifi M. Increasing the production of plant secondary metabolites using biotic elicitors. Journal of Cell & Tissue, 2013; 4(2):119-128.
[6]. Larcher W. Physiological Plant Ecology. Springer-Verlag. New York. 1995.
[7]. Ali A, Maher S, Khan SA, Chaudhary MI, Musharraf SG. Sensitive quantification of six steroidal lactones in Withania coagulans extract by UHPLC electrospray tandem mass spectrometry. Steroids. 2015; 104:176-181.
[8]. Prasad SK, Kumar R, Patel DK, Hemalatha S, Wound healing activity of Withania coagulansin streptozotocininduced diabetic rats, Pharm. Biol. 2010; 48: 1397–1404.
[9]. Gamoh K, Hirayama M, Ikekawa N. Stereocontrolled synthesis of Withanolide D and related compounds. Journal of the Chemical Society, Perkin Transactions. 1984; 1:449-54.
[10]. Kannan N, Kulandaivelu G. Novel method to isolate Withaferin A from Withania somnifera roots and its bioactivity. Allelopathy Journal. 2007; 20(1): 213-20.
[11]. Sangwan R, Chaurasiya N, Misra L, Lal P, Uniyal G, Sharma R, et al. Phytochemical variability in commercial herbal products and preparations of Withania somnifera (Ashwagandha). Current Science. 2004; 10: 461-5.
[12]. Sunohara Y, Matsumoto H. Oxidative injury induced by the herbicide quinclorac on Echinochloa oryzicola Vasing and the involvement of antioxidative ability in its highly selective action in grass species. Plant Science. 2004; 167(3): 597-606.
[13]. Khunpon B, Suriyan C-U, Faiyue B, Uthaibutra J, Saengnil K. Regulation on antioxidant defense system in rice seedlings (Oryza sativa L. ssp. indica cv.‘Pathumthani 1’) under salt stress by paclobutrazol foliar application.Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2019; 47(2): 368-77.
[14]. Shahverdi MA, Omidi H, Tabatabaei SJ. Stevia (Stevia rebaudiana Bertoni) responses to NaCl stress: Growth, photosynthetic pigments, diterpene glycosides and ion content in root and shoot. Journal of the Saudi Society of Agricultural Sciences, 2019; 18:355-360.
[15]. Aghighi Shahverdi M, Omidi H, Tabatabaei SJ. Effect of nutri-priming on germination indices and physiological characteristics of stevia seedling under salinity stress. Journal of Seed Science. 2017; 39:353-362.
[16]. Blainski A, Lopes G, de Mello J. Application and analysis of the folin ciocalteu method for the determination of the total phenolic content from Limonium brasiliense L. Molecules. 2013; 18(6): 6852-65.
[17]. Maghsoudi Moud A, Heravi M. Salt stress effects on seedling growth rate and sodium, potassium, and calcium ions content of wheat (Triticum aestivum L.) cultivars. JCPP. 2015; 5(15):115-128.
[18]. Afshar Mohamadian A., Omidipour M. and Jamal Omidi F. Effect of different drought stress levels on content and chlorophyll fluorescence indices of two bean cultivars (Phaseolus vulgaris L.). Journal of Plant Research, 2019; 31(3): 511-525.
[19]. Enders ALJ. Comparison of wet-digestion and dry-ashing methods for total elemental analysis of biochar. Communications in Soil Science and Plant Analysis. 2012; 43(7): 1042-52.
[20]. Aghighi Shahverdi M, Omidi H, Tabatabaei SJ. Stevia (Stevia rebaudiana Bertoni) responses to NaCl stress: Growth, photosynthetic pigments, diterpene glycosides and ion content in root and shoot. Journal of the Saudi Society of Agricultural Sciences. 2019; 18(4):355-360.
[21]. Abdeshahian M, Nabipour M, Meskarbashee M. Chlorophyll fluorescence as criterion for the diagnosis salt stress in wheat (Triticum aestivum) plants. Int J Chem Biol Eng. 2010; 4: 184-6.
[22]. Chai Q, Gan Y, Zhao C, Xu HL, Waskom RM, Niu Y, Siddique KH. Regulated deficit irrigation for crop production under drought stress. A review. Agronomy for Sustainable Development. 2016; 36: 1-21. [23]. Biglouei M, Assimi M, Akbarzadeh A. Effect of water stress at different growth stages on quantity and quality traits of Virginia (flue-cured) tobacco type. Plant, Soil and Environment. 2010; 56(2): 67-75. [24]. Singh R, Gupta P, Khan F, Singh SK, Mishra T, Kumar A, et al. Modulations in primary and secondary metabolic pathways and adjustment in physiological behaviour of Withania somnifera under drought stress. Plant Science. 2018; 272: 42-54.
[25]. Agarwal S, Pandey V. Antioxidant enzyme resposes to NaCl stress in Cassia angustifolia. Biology Plant. 2004; 48: 555-560.
[26]. 26. Cavalcanti FR, Oliveira JT, Martins-Miranda A, Viegas AS, Silveira RA. Superoxide dismutase, catalase and peroxidase activities do not confer protection against oxidative damage in saltstressed cowpea leaves. New Phytol. 2004; 163: 563-571.
[27]. Mittler R. Oxidative stress, antioxidant and stress tolerance. Trend in Plant Science. 2002; 7(9): 405-415.
[28]. 28. Rukmini MS, Benedicta DS, Vivan DS. Superoxide dismutase and catalase activities and their correlation with malon dialdehyde in schizophrenic patients. Clinical Biochemistery. 2004; 19: 114-118. [29]. Hameed A, Goher M, Iqbal N. Drought induced programmed cell death and associated changes in antioxidants, proteases and lipid peroxidation in wheat leaves. Biologia Plantarum. 2013; 57(2): 370-374. [30]. Reddy AR, Kolluru VC, Munusamy V. Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants. Journal of Plant Physiology 2004; 11: 1189-1202.
[31]. Sharma P, Jha AB, Dubey SS, Pessarakli M. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of Botany, 2012; 26:1-10. [32]. André CM, Schafleitner R, Legay S, Lefèvre I, Aliaga CAA, Nomberto G, Hoffmann L, Hausman JF, Larondelle Y, Evers D. Gene expression changes related to the production of phenolic compounds in potato tubers grown under drought stress. Phytochemistry. 2009; 70(9): 11071116.
[33]. Blasco BA, Leyva R, Romero L, Ruiz JM. Iodine effects on phenolic metabolism in lettuce plants under salt stress.Journal of Agricultural and Food Chemistry. 2013; 61:25912596.
[34]. Halliwell B, Gutteridge JM. Free radicals in biology and medicine. Oxford University Press, USA. 2015.
[35]. Farooq MWA, Kobayashi N, Fujita D, Basra SM. Plant drought stress: effects, mechanisms and management. Agron. Sustain Dev. 2009; 29: 185-212.
[36]. Jiang Y, Huang B. Protein alternations in tall fescue in response to drought stress and abscisic acid. Crop Science. 2002; 42: 202-207.
[37]. Rahdari P, Hoseini SM. Drought stress: a review. International Journal of Agronomy and Plant Production. 2012; 3: 443-446.
[38]. Nair AS, Abraham T, Jaya D. Studies on the changes in lipid peroxidation and antioxidants in drought stressinduced cowpea (Vigna unguiculata L.) varieties. Journal Environment Biology. 2008; 29: 689-691.
[39]. Perry TW, Rhykerd CL, Holt DA, Mayo HH. Effect of potassium fertilization on chemical characteristics, yield and nutritive value of corn silage. Journal of Animal Science. 2011; 34: 642-646. [40]. Tilahun A, Sven S. Mechanisms of drought resistance in grain: PSII stomatal regulation and root growth. Ethiopian Journal of Science. 2003; 26: 137-144.
[41]. Li GL, Wu HX, Sun YQ, Zhang SY. Response of chlorophyll fluorescence parameters to drought stress in sugar beet seedlings. Russian Journal of Plant Physiology. 2013; 60: 337–342.
دوره 28، شماره 2
خرداد و تیر 1400
صفحه 274-285
  • تاریخ دریافت: 03 دی 1398
  • تاریخ بازنگری: 14 فروردین 1399
  • تاریخ پذیرش: 17 فروردین 1399
  • تاریخ اولین انتشار: 01 خرداد 1400