عنوان مقاله [English]
نویسندگان [English]چکیده [English]
Background: The aim of this study was to evaluate the effects of Elaeagnus Angustifolia extract on mouse embryonic development of Balb/c.
Materials and Methods: Thirty pregnant mice were randomly divided into two groups. Control group consumed drinking water, and the experimental group during pregnancy received the aqueous extract at a dose of 500 mg/ kg. Pregnant mice were killed at 18th gestation day and fetal and placental weight and length of the crown-rump of fetuses were measured and recorded. After fixation and tissue processing, liver embryos were taken out and tissue sections were prepared. After staining with hematoxylin-eosin, histomorphometry was investigated. Then obtained results were statistically analyzed.
Results: No apparent abnormality was observed in embryos. The mean of crown-rump length of fetuses in the experimental group was not shown a significantly increased than control group. The mean of fetal weight in the experimental group was significantly decreased than control group. The mean weight of the placenta in the experimental group was significantly reduced compared to control group. In a histomorphomety analysis in the embryo liver of experimental group, with regard to the mean of size percentage of sinusoids and mean number of blood cells significantly increased in experimental group compared to control group. The mean total surface that occupied by hepatocyte and Mean number of hepatocyte nuclei significantly decreased in experimental group compared to control group.
Conclusion: It seems prescribing aqueous extract of Elaeagnus Angustifolia at dose of 500 mg/kg to pregnant mice can cause developmental abnormalities in the fetus and placenta.