بررسی اثرات ضدمیکروبی عصاره اتانولی آویشن باغی در برابر باکتری‌های بیماری‌زای انسانی

نویسندگان

1 کارشناس ارشد، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران.

2 استادیار، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران.

3 کارشناس ارشد، گروه اصلاح نباتات و بیوتکنولوژی، پژوهشکده زیست‌فناوری کشاورزی، دانشگاه زابل، زابل، ایران.

چکیده

اهداف هدف از این مطالعه بررسی اثرات ضدمیکروبی عصاره اتانولی آویشن باغی در برابر پاتوژن‌های مهم انسانی است.
مواد و روش ها عصاره گیاهی آویشن با استفاده از دستگاه خلأ از مرکز (روتاری) انجام شد. باکتری به صورت استاندارد تهیه شد که شامل Streptococcus pyogenes ATCC® 19615™, Streptococcus pneumoniae ATCC 49619, S.Saprophyticus ATCC® 15305, Hafnia alvei ATCC 51873, Acinetobacter. baumannii ATCC 19606, Enterococcus faecalis ATCC 29212, Proteus mirabilis ATCC 35659, Serratia marcescens ATCC 274, Staphylococcus aureus ATCC® 25923 است. حداقل غلظت بازدارندگی عصاره آویشن در غلظت‌های مختلف با روش رقت‌سازی در چاهک روی باکتری‌ها تعیین شد. حساسیت سویه‌ها به چند آنتی‌بیوتیک با روش استاندارد دیسک دیفیوژن کربی‌بائر ارزیابی شد.
یافته ها نتایج نشان داد عصاره برگ آویشن در همه غلظت‌ها باکتری‌ها را مهار می‌کند. کمترین غلظت مهارکنندگی در برابرS.pneumoniae, H.alvei, P.mirabilis و S.marcescens مشاهده شده است (25ppm/6).
نتیجه گیری نتایج این مطالعه نشان‌دهنده تأثیر زیاد عصاره الکلی روی باکتری‌های بیماری‌زا است. البته کاربرد بالینی این گیاهان نیازمند مطالعات بیشتر و وسیع‌تری است.

کلیدواژه‌ها


عنوان مقاله [English]

Assessment of the Antimicrobial Effects of the Vulgaris Thymus Ethanol Extract Against Human Pathogenic Bacteria

نویسندگان [English]

  • Ali Asghar Ghaderi 1
  • Baratali Fakheri 2
  • Nafise Mahdinezhad 2
  • Saeede Saeedi 3
1 MSc., Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran.
2 Assistant Professor, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Iran.
3 MSc., Department of Plant Breeding and Biotechnology, Agricultural Biotechnology Institute, University of Zabol, Zabol, Iran.
چکیده [English]

Backgrounds The current study aimed at investigating the antimicrobial effects of Vulgaris thymus ethanol extract on the important human pathogens.
Methods & Materials The thymus alcoholic extract was provided based on a standard protocol. The following standard bacteria were also provided: Streptococcus pyogenes ATCC 19615, Streptococcus pneumoniae ATCC 49619, S. saprophyticus ATCC15305, Hafnia alvei ATCC 51873, Acinetobacter baumannii ATCC 19606, Enterococcus faecalis ATCC 29212, Proteus mirabilis ATCC 35659, Serratia marcescens ATCC 274, Staphylococcus aureus ATCC 25923.
The minimum inhibitory concentration (MIC) of thymus ethanol extract on the bacteria was determined. The linear sensitivity of the bacteria against some antibiotics was also measured by the standard method of Kirby-Bauer disk diffusion.
Results The results showed that the thymus leaf alcoholic extract inhibited the bacterial growth in the evaluated concentrations. The MIC of 6/25 ppm was observed effective against S. pneumoniae, H. alvei, P. mirabilis, and S. marcescens.
Conclusion The results of the current study indicated the high effect of thymus alcoholic extract on the pathogenic bacteria. However, the medicinal application of plants requires further investigations.

کلیدواژه‌ها [English]

  • Vulgaris thymus
  • Antimicrobial activity
  • Ethanol extract
  • Human pathogenic bacteria
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