بررسی ترکیبات فنلی و ارزیابی ظرفیت آنتی‌اکسیدانی و ضدالتهابی عصاره الکلی دو گیاه ناخنک و زبان گنجشک

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی ارشد، گروه مهندسی شیمی و نفت، دانشگاه آزاد اسلامی، واحد قوچان، قوچان، ایران.

2 استادیار، گروه مهندسی شیمی و نفت، واحد قوچان، دانشگاه آزاد اسلامی، قوچان، ایران.

چکیده

اهداف در سال‌های اخیر، یافتن ترکیبات جدید با منشأ گیاهی در درمان و پیشگیری از بیماری‌ها، مدنظر محققان قرار گرفته است. هدف مطالعه حاضر بررسی توان آنتی‌اکسیدانی و ضدالتهابی گیاهان ناخنک و زبان گنجشک است که در طب سنتی استفاده می‌‌شود.
مواد و روش ها در این پژوهش، پس از تهیه عصاره اتانولی گیاهان ناخنک و زبان گنجشک به روش خیساندن، میزان توان آنتی‌اکسیدانی گیاهان با روش ارزیابی احیای یون مس و قدرت ضدالتهابی آن‌ها با آزمون ممانعت از تغییر شکل پروتئین آلبومین گاوی، اندازه‌گیری شد.
یافته ها نتایج نشان داد هر دو گیاه اثر آنتی‌اکسیدانی و ضدالتهابی دارند. فعالیت آنتی‌اکسیدانی و ضدالتهابی به غلظت عصاره‌ها وابسته است و با افزایش غلظت عصاره‌ها، خاصیت آنتی‌اکسیدانی و ضدالتهابی آن‌ها افزایش می‌یابد. بیشترین میزان ترکیبات فنلی در هر گرم عصاره خشک گیاه زبان گنجشک 047/0 میلی‌گرم است. این گیاه بیشترین اثر آنتی‌اکسیدانی (045/0±714/0) را دارد. بیشترین میزان ترکیبات فلاونوئیدی در گیاه ناخنک 23/9 میلی‌گرم است. این گیاه بیشترین اثر ضدالتهابی ( 033/0±217/0) را دارد.
نتیجه گیری استفاده از روش‌های غربالگری همانند روش‌های ذکرشده، فرصتی برای یافتن ترکیبات فعال با چند اثر متفاوت را فراهم خواهد کرد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of Phenolic Compounds and Assessment of Antioxidant Capacity and Anti-Inflammatory of Alcoholic Extraction of Melilotus Officinalis and Fraxinus Excelsior

نویسندگان [English]

  • Fariba Zarei 1
  • Susan Khosroyar 2
1 MSc. Student, Department of Chemical and Petroleum Engineering, Islamic Azad university, Quchan Branch, Quchan, Iran.
2 Assistant Professor, Department of Chemical and Petroleum Engineering, Quchan Branch, Islamic Azad University, Quchan, Iran.
چکیده [English]

Objectives In recent years, researchers tried to find new compounds with herbal origins to treat and prevent diseases. The current study aimed at investigating the antioxidant and anti-inflammatory effects of Melilotus officinalis and Fraxinus excelsior alcoholic extracts, commonly used in traditional medicines.
Methods After preparing the ethanolic extracts of M. officinalis and F. excelsior by the maceration method, the antioxidant properties were investigated by the cupric ion reducing assay (cupric assay) and the anti-inflammatory effects were also investigated through the prevention test of bovine serum albumin (BSA) and its transformation potential.
Results The obtained results showed that both mentioned plants had antioxidant and anti-inflammatory effects. The antioxidant properties changed based on the concentration of extracts, and antioxidant and anti-inflammatory properties increased along with the concentration increase. The highest amount of phenolic compound in F. excelsior was 0.04 mg/g of dry extract; this plant also showed the highest antioxidant effect (0.714±0.045). The highest amount of flavonoid compounds in M. officinalis was 9.23 mg; this plant showed the highest anti-inflammatory effects (0.21±0.033 ).
Conclusion The employed screening methods were useful to find biological components with different properties.

کلیدواژه‌ها [English]

  • Antioxidant
  • Anti-inflammatory
  • Melilotus officinalis
  • Fraxinus excelsior
Cowan MM. Plant products as antimicrobial agents. Clinical Microbiology Reviews. 1999; 12(4):564-82. PMCID: PMC88925
Magaji MG, Anuka JA, Abdu-Aguye I, et al. models. JMPR .2008; 2: 39- 44.
Huang ZR, Lin YK, Fang JY. Biological and pharmacological activities of squalene and related compounds: potential uses in cosmetic dermatology. Molecules. 2009; 14(1):540–54. doi: 10.3390/molecules14010540
Asghari S, Naderi GH, Bashardoost N, Etminan Z. The study of antioxidant potential of chamaemelum nobile extract on liver cell of rats. Journal of Herbal Drugs. 2011; 1:69-76.
Koksal E, Gulcin I. Antioxidant activity of cauliflower. Turkish Journal of Agriculture and Forestry. 2008; 32(1):65-78.
Zihreh F, Nasri S, Karishchi P. [The effect of quercetin on pituitary–gonadal axis, sperm parameters and testis tissue in male rats(Persian)]. Journal of Sabzevar University of Medical Sciences. 2015; 22 (3) :377-386.
Kähkönen MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja K, Kujala TS, et al. Antioxidant activity of plant extracts containing phenolic compounds. Journal of Agricultural and Food Chemistry. 1999; 47(10):3954–62. doi: 10.1021/jf990146l
Anilkumar M. Ethnomedicinal plants as anti-inflammatory and analgesic agents. In: Chattopadhyay D. Ethnomedicine: A Source of Complementary Therapeutics. New York: Barnes & Noble; 2010.
Homayoni Tabrizi M, Asoodeh A, Shabestarian H. [Isolation and identification of a new antioxidant peptide from casein camel milk using pepsin and pancreatin (Persian)]. Journal of Sabzevar University of Medical Sciences. 2015; 22(1):45-56.
Palasuwan A, Soogarun S, Lertlum T, Pradniwat P, Wiwanitkit V.Inhibition of heinz body induction in an in vitro model and total antioxidant activity of medicinal Thai plants. Asian Pacific Journal of Cancer Prevention. 2006; 6(1):458-463.
Ramman N. Phytochemical techniques. New Delhi: India Publishing Agency; 2006
Adedapo AA, Jimoh FO, Koduru S, Afolayan AJ, Masika PJ. Antibacterial and antioxidant properties of the methanol extracts of the leaves and stems of Calpurnia aurea. BMC Complementary and Alternative Medicine. 2008; 8:53. doi: 10.1186/1472-6882-8-53
Taylor RSL, Edel F, Manandhar NP, Towers GHN. Antimicrobial activities of southern Nepalese medicinal plants. Journal of Ethnopharmacology. 1996; 50(2):97–102. doi: 10.1016/0378-8741(95)01335-0
Apak R, Güçlü K, Özyürek M, Çelik SE. Mechanism of antioxidant capacity assays and the CUPRAC (cupric ion reducing antioxidant capacity) assay. Microchimica Acta. 2007; 160(4):413–9. doi: 10.1007/s00604-007-0777-0
Apak R, Güçlü K, Özyürek M, Çelik SE. Mechanism of antioxidant capacity assays and the CUPRAC (cupric ion reducing antioxidant capacity) assay. Microchimica Acta. 2007; 160(4):413–9. doi: 10.1007/s00604-007-0777-0
Sakat S, Juvekar AR, Gambhire MN. In vitro antioxidant and antiinflammatory activity of methanol extract of oxalis corniculata Linn. International Journal of Pharmacy and Pharmaceutical Sciences 2010; 2(1):146-155.
Hayouni E, Abedrabba M, Bouix M, Hamdi M. The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. and Juniperus phoenicea L. fruit extracts. Food Chemistry. 2007; 105(3):1126–34. doi: 10.1016/j.foodchem.2007.02.010
Mita S, Murano N, Akaike M, Nakamura K. Mutants of Arabidopsis thaliana with pleiotropic effects on the expression of the gene for beta-amylase and on the accumulation of anthocyanin that are inducible by sugars. Plant Journal. 1997; 11(4):841–51. doi: 10.1046/j.1365-313x.1997.11040841.x
Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis. 2002; 10(3):178-182.
Koksal E, Gulcin I. Antioxidant activity of cauliflower. Turkish Journal of Agriculture and Forestry. 2008; 32:65-78.
Apak R, Güçlü K, Özyürek M, Bektas¸oğlu B, Bener M. Cupric ion reducing antioxidant capacity assay for food antioxidants: vitamins, polyphenolics, and flavonoids in food extracts. Advanced Protocols in Oxidative Stress I. 2008; 163–93. doi: 10.1007/978-1-60327-517-0_14
Johnsy G, Kaviyarasan V. Antimicrobial and antioxidant properties of trametes gibbosa. Journal of Pharmacy Research. 2011; 4(11): 3939-3942.
Pleşca-Manea L, Pârvu AE, Pârvu M, Taaˇmaş M, Buia R, Puia M. Effects ofMelilotus officinalison acute inflammation. Phytotherapy Research. 2002; 16(4):316–9. doi: 10.1002/ptr.875
Braga P, Sasso M, Lattuada N, Marabini L, Calo R, Antonacci R, et al. Antioxhdant activity of Melilotus officinalis extract investigated by mean of the radical Scavenging activity ,the chemilu minescence of human neutrophil bursts and lipoperoxidation assay. Journal of Medicinal Plants Research. 2013; 7(7):358-365
Krzakowa M, Grzywacz E. Phenolic compounds pattern in sweet clover (Melilotus officinalis) vs white clover (M. alba) revealed by 2D TLC (two-dimentional thin-layer chromatography) and its taxonomic significance. Herba Polonica. 2010; 56(3): 53-62.
Middletona P, Stewart F, Al-Qahtani S, Egan P, Rourke C, Abdulrahman A. Antioxidant, antibacterial activities and general toxicity of alnus glutinosa, fraxinus excelsior and Papaver Rhoeas. Iranian Journal of Pharmaceutical Research. 2005; 2:81-86.
Bai N, He K, Ibarra A, Bily A, Roller M, Chen X, et al. Iridoids fromfraxinus excelsiorwith adipocyte differentiation-inhibitory and pparα activation activity. Journal of Natural Products. 2010; 73(1):2–6. doi: 10.1021/np9003118
Waterman PG, Mole S. Analysis of phenolic plant metabolites. Oxford: Blackwell Scientific Publications; 1994.
Elmastaş M, Dermirtas I, Isildak O, Aboul-Enein HY. Antioxidant activity of s-carvone isolated from spearmint (Mentha Spicata L. Fam Lamiaceae). Journal of Liquid Chromatography & Related Technologies. 2006; 29(10):1465–75. doi: 10.1080/10826070600674893
Bunney, S. The illustrated encyclopedia of herb. Their medicinal and culinary uses. Carpenter, RC., Sothesswaran: Chanceller Press London; 1992.